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In order to calculate the ***significance*** of the difference between a ***control sample*** and a sample undergone some ***treatment*** in an DGE experiment it is strongly advisable to have replicates for the control and the treated sample. Based on an experiment in yeast, 6 replicated would be a minimum.
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In many experiment 3 replicates are used, being a comprise between power and cost.
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If the cost of replicates is low compared to libaray preparation, replicates can be pools of multiple samples as described in this publication: https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6721-y
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If the cost of replicates is low compared to library preparation, replicates can be pools of multiple samples as described in this publication: https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6721-y
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[Scotty](http://scotty.genetics.utah.edu/) |
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[Scotty is a tool for Power Analysis for RNA Seq Experiments](http://scotty.genetics.utah.edu/) |
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