In order to calculate the ***significance*** of the difference between a ***control sample*** and a sample undergone some ***treatment*** in an DGE experiment it is strongly advisable to have replicates for the control and the treated sample. Based on an experiment in yeast, 6 replicated would be a minimum.
In order to calculate the ***significance*** of the difference between a ***control sample*** and a sample undergone some ***treatment*** in an DGE experiment it is strongly advisable to have replicates for the control and the treated sample. Based on an experiment in yeast, 6 replicated would be a minimum.
In many experiment 3 replicates are used, being a comprise between power and cost.
In many experiment 3 replicates are used, being a comprise between power and cost.
If the cost of replicates is low compared to libaray preparation, replicates can be pools of multiple samples as described in this publication: https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6721-y
