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How many **replicates** do I need for an RNA differential expression analyse?
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**How many **replicates** do I need for an RNA differential expression analyse?**
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In order to calculate the ***significance*** of the difference between a ***control sample*** and a sample undergone some ***treatment*** in an DGE experiment it is strongly advisable to have replicates for the control and the treated sample. Based on an experiment in yeast, 6 replicated would be a minimum.
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In many experiment 3 replicates are used, being a comprise between power and cost.
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