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**Can RNA be sequenced?**
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On [Illumina](faq_NGStechnologies) type sequencers, RNA hast to be converted to cDNA first. Depending on the size of the RNA, a fractionation step is needed to reduce the size of the cDNA to be compatible with the cluster formation (20 to 1000bp).
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On an Oxford Nanopre sequencer, both cDNA and also RNA can be directly sequenced if the RNA is available in a sufficient amounts.
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When sequencing RNA on oxford nanopore modifications bases can be detected. The detection of RNA modifications is still under full development. |
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