NGS (Next generation sequencing)

NGS is a fast changing technology. Two complementary technologies are used at the genomic platform. Short read sequencing is based on Illumina sequencers and for many questions the answers given is based on this technology. Long read molecules are generated on Oxford Nanopore flow cells. Based on these NGS technologies a broad spectrum of applications is available from de novo assemblies to spatial transcriptomics ...

Please contact the genomics platform if you need information not covered by this faq.

• Which high throughput sequencing technologies are offered by the GIGA genomics platform and how do they work?

• Can RNA be sequenced?

• What type of RNA molecules can be sequenced?

• What do I need to provide to the platform to prepare a standard Illumina TruSeq mRNA library?

• What do I need to provide to the platform to prepare Illumina mRNA stranded ligation library

• What is the minimum amount of RNA needed to make an RNA seq experiment?

• What do I need to provide to the platform to prepare a standard Illumina TruSeq totalRNA library?

• In what format do I receive the results from sequencing?

• How many short reads do I need to per samples to do a RNA seq analysis?

• I want to do a RNA differential expression analyses (RNA-seq DGE). What do I need?

• How many replicates do I need for an RNA differential expression analyse?

• When do I use Sanger sequencing and when Illumina NGS sequencing?

• How to measure RNA quality and quantity?

• What do I need to provide to the platform to prepare a standard Takara SMART seq Ultra low input library?

• How to use Qiazol to collect sorted cells for RNA extraction?

• When Do I Use Sanger Sequencing vs NGS??

• Can I sequence and assemble a genome??

• Single cell transcriptome sequencing

• Illumina adapters, indexes and primers