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**How to measure RNA quality and quantity?**
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**RNA/DNA purity**
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The ratio of absorbance 260/280nm is used to assess the purity of DNA and RNA.
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A ratio of ~1.8 is generally accepted as “pure” for DNA; a ratio of ~2.0 is generally accepted as “pure” for RNA. Lower ratio may indicate the presence of protein, phenol or other contaminants that absorb strongly at or near 280 nm.
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The ratio of absorbance 260/230 nm for “pure” nucleic acid are often in the range of 2.0-2.2. If the ratio is appreciably lower than
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expected, it may indicate the presence of contaminants (EDTA, carbohydrates, phenol,TRIzol reagent,...)
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**RNA quality**
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RIN score /DIV
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[**evaluating-rna**](https://www.illumina.com/content/dam/illumina-marketing/documents/products/technotes/evaluating-rna-quality-from-ffpe-samples-technical-note-470-2014-001.pdf)
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